Death-Associated Protein 3 (DAP3) antibody

Antigen: Death-Associated Protein 3 (DAP3)

Synonyms: DAP6, EAP1, BING2, MGC126245, MGC126246, DAP-3, 4921514D13Rik, MRPS29, MRP-S29, bMRP-10, MGC126058, MGC126059, DKFZp686G12159, DAP3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184743

Additional Information

Alternative name: DAP3

Gene ID: 7818

Immunogen: NPSLLERHCAYL

Description: All names and symbols: DAP3, death associated protein 3, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29, bMRP-10, death-associated protein 3, mitochondrial 28S ribosomal protein S29. Official Symbol: DAP3. RefSeq number(s): NP_387506.1, NP_004623.1. Gene ontology terms: structural constituent of ribosome, induction of apoptosis by extracellular signals, apoptosis, mitochondrial ribosome, small ribosomal subunit, mitochondrion

Specificity: This antibody is expected to recognise both human isoforms of this protein. Human 398 aa, 398 aa

Application Details

Application Notes: Western blot: Approx 40-45kDa band seen in HeLa lysate. Recommended for use at 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Apoptosis/Necrosis, DNA/RNA

Restrictions: For Research Use only

Images

ABIN184743 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Kissil, Deiss, Bayewitch et al.: "Isolation of DAP3, a novel mediator of interferon-gamma-induced cell death." in: The Journal of biological chemistry, Vol. 270, Issue 46, pp. 27932-6, 1996 (PubMed).