Drebrin-Like (DBNL) antibody

Antigen: Drebrin-Like (DBNL)

Synonyms: Abp1, SH3P7, mAbp1, ABP1, HIP-55, Sh3p7, dbnl, dbnl-A, MGC53920, wu:fb34f02, dbnl-B, MGC84047, DBNL, MGC166201, MGC112450, DKFZp459C0939

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184747

Additional Information

Alternative name: HIP-55 / SH3P7

Gene ID: 28988, 13169 (mouse), 83527 (rat)

Immunogen: AANLSRNGPALQE-C

Format: Affinity-purified

Description: All names and symbols: HIP-55, SH3P7, src homology 3 domain-containing protein HIP-55, HIP-55 protein, drebrin F. Official Symbol: DBNL. RefSeq number(s): NP_054782.2, NP_001014436.1. Gene ontology terms: enzyme activator activity, protein binding activity, activation of JUN kinase

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1/32,000. Western Blot: Approx 55kDa band observed in Jurkat and 3T3 lysates (predicted MW of 49kDa according to NP_054782). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184747 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Ensenat, Yao, Wang et al.: "A novel src homology 3 domain-containing adaptor protein, HIP-55, that interacts with hematopoietic progenitor kinase 1." in: The Journal of biological chemistry, Vol. 274, Issue 48, pp. 33945-50, 1999 (PubMed).