PAFAH1B1 antibody (C-Term)

Catalog No. ABIN184751

The Goat Polyclonal anti-PAFAH1B1 antibody is suitable to detect PAFAH1B1 in samples from Human and Rat. It has been validated for WB, ELISA and IHC.

Quick Overview for PAFAH1B1 antibody (C-Term) (ABIN184751)

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Reactivity: Human, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This PAFAH1B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-PAFAH1B1 Antibody

Binding Specificity: C-Term

Purpose: LIS1 / PAFAH1B1

Sequence: TGSVDQTVKV WECR

Cross-Reactivity: Human, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence TGSVDQTVKVWECR, from the C Terminus of the protein sequence according to NP_000421.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Brain (Cortex), Thyroid and Pancreas. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 40 kDa band observed in Human Lung and Brain (Cerebellum) and in Rat Ovary lysates (calculated MW of 46.6 kDa according to Human NP_000421.1 and to Rat NP_113951.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAFAH1B1

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Alternative Name: PAFAH1B1

Background: PAFAH1B1, LIS1, platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45 kDa, MDCR, PAFAH, lissencephaly 1 protein, Platelet-activating factor acetylhydrolase, isoform 1B, alpha subunit, platelet-activating factor acetylhydrolase, isoform I

Gene ID: 5048

NCBI Accession: NP_000421

Pathways: M Phase, Regulation of Cell Size