GULP, Engulfment Adaptor PTB Domain Containing 1 (GULP1) antibody

Antigen: GULP, Engulfment Adaptor PTB Domain Containing 1 (GULP1)

Synonyms: CED6, GULP, CED-6, FLJ31156, Ced6, Gulp-2, 3110030A04Rik, 5730529O06Rik, GULP1, fj61e02, MGC123057, wu:fj61e02, si:by35d8.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184754

Additional Information

Alternative name: GULP1 / CED6

Gene ID: 51454, 70676 (mouse)

Immunogen: EGTVFCLDPLDSRC

Format: Affinity-purified

Description: All names and symbols: CED-6, GULP1, CED-6 protein, GULP, engulfment adaptor PTB domain containing 1. Official Symbol: GULP1. RefSeq number(s): NP_057399.1. Gene ontology terms: apoptosis, signal transduction, phagocytosis, engulfment

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 35kDa band observed in Human Placenta lysates (calculated MW of 34.5kDa according to NP_057399.1). Recommended concentration: 0.2-0.6µg/ml. An additional band of unknown identity was also consistently observed at approx.46kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration, 5-10 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184754 staining (0.2µg/ml) of Human Placenta lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184754 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Su, Nakada-Tsukui, Tosello-Trampont et al.: "Interaction of CED-6/GULP, an adapter protein involved in engulfment of apoptotic cells with CED-1 and CD91/low density lipoprotein receptor-related protein (LRP)." in: The Journal of biological chemistry, Vol. 277, Issue 14, pp. 11772-9, 2002 (PubMed).