Flotillin 1 (FLOT1) antibody

Antigen: Flotillin 1 (FLOT1)

Synonyms: FLOT1, DKFZp469E0515, flot1, Rareg, flot1c, MGC84970, MGC132244, flotillin1, flot1a, flotillin-1, MGC143379, reggie2, wu:fj45g11, si:dz44o19.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184756

Additional Information

Alternative name: Flotillin 1

Gene ID: 10211, 14251 (mouse)

Immunogen: C-SISQVNHKPLRTA

Format: Affinity-purified

Description: All names and symbols: FLOT1, flotillin 1, reggie-2, integral membrane component of caveolae. Official Symbol: FLOT1. RefSeq number(s): NP_005794. Gene ontology terms: caveolae, integral membrane protein

Specificity: Human, Dog, Cow, Pig

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 48kDa band observed in H460 lysates (predicted MW of 49kDa according to NP_005794). Recommended for use at 0.2-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cytoskeleton, Microfilaments

Restrictions: For Research Use only

Images

ABIN184756 staining (0.3µg/ml) of H460 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Bickel, Scherer, Schnitzer et al.: "Flotillin and epidermal surface antigen define a new family of caveolae-associated integral membrane proteins." in: The Journal of biological chemistry, Vol. 272, Issue 21, pp. 13793-802, 1997 (PubMed).