E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) antibody

Antigen: E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1)

Synonyms: zef1, fb05d07, wu:fb05d07

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184790

Additional Information

Alternative name: ELF1

Gene ID: 1997, 13709 (mouse)

Immunogen: C-AMKQNELLEPNSF

Format: Affinity-purified

Description: All names and symbols: ELF1, E74-like factor 1 (ets domain transcription factor). Official Symbol: ELF1. RefSeq number(s): NP_758961.1. Gene ontology terms: nucleus, regulation of transcription, DNA-dependent, transcription factor activity,

Specificity: Human, Mouse 619

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in Human Tonsil, Lymph Node and in Jurkat T-cell line lysates (calculated MW of 67.5kDa according to NP_758961.1). Recommended concentration: 0.3-1.0µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184790 staining (0.3µg/ml) of Human Tonsil lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Wang, Petryniak, Thompson et al.: "Regulation of the Ets-related transcription factor Elf-1 by binding to the retinoblastoma protein." in: Science (New York, N.Y.), Vol. 260, Issue 5112, pp. 1330-5, 1993 (PubMed).