Splicing Factor 3b, Subunit 3, 130kDa (SF3B3) antibody

Antigen: Splicing Factor 3b, Subunit 3, 130kDa (SF3B3)

Synonyms: RSE1, SAP130, SF3b130, STAF130, KIAA0017, MGC139814, rse1, sap130, sf3b130, staf130, NV14176, DKFZp469G1915

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184798

Additional Information

Alternative name: SAP130 / SF3B3

Gene ID: 23450, 101943 (mouse)

Immunogen: C-VSKKLEDIRTRYAF

Description: All names and symbols: SF3B3, SAP130, splicing factor 3b, subunit 3, 130kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3. Official Symbol: SF3B3. RefSeq number(s): NP_036558.3. Gene ontology terms: pre-mRNA splicing factor activity, small nuclear ribonucleoprotein, mRNA processing, mRNA splicing, protein complex assembly, spliceosome complex

Specificity: Human, Mouse, Rat, Dog 1217 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184798 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Das, Xia, Palandjian et al.: "Characterization of a protein complex containing spliceosomal proteins SAPs 49, 130, 145, and 155." in: Molecular and cellular biology, Vol. 19, Issue 10, pp. 6796-802, 2000 (PubMed).