ADP-Ribosylation Factor-Like 4A (ARL4A) antibody

Antigen: ADP-Ribosylation Factor-Like 4A (ARL4A)

Synonyms: arl4a, MGC80459, MGC89206, ARL4A, MGC127610, Arl4a, ACYPI003705, ARL6, ARF4L, ARL4, Arl5, Arf4l, Arfl4, AW456149, MGC28743, 1110036H21Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184801

Additional Information

Alternative name: ARL4A

Gene ID: 10124, 11861 (mouse)

Immunogen: GNGLSDQTSILSN-C

Format: Affinity-purified

Description: All names and symbols: ARL4A, ARL4, ADP-ribosylation factor-like 4, ADP-ribosylation factor-like 4A. Official Symbol: ARL4A. RefSeq number(s): NP_005729. Gene ontology terms: small monomeric GTPase activity, GTP binding, small GTPase mediated signal transduction, nucleus

Specificity: This is also expected to react with a hypothetical human protein, XP_166703, and a hypothetical mouse protein, AAH03725 - both very similar to ARL4. Human, Mouse, Rat 200 aa

Application Details

Application Notes: Western blot: Approx 23kDa band seen in Human Testis lysate. Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184801 staining (2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Schuermann, Breiner, Becker et al.: "Cloning of two novel ADP-ribosylation factor-like proteins and characterization of their differential expression in 3T3-L1 cells." in: The Journal of biological chemistry, Vol. 269, Issue 22, pp. 15683-8, 1994 (PubMed).

Jacobs, Schuermann, Becker et al.: "The mouse ADP-ribosylation factor-like 4 gene: two separate promoters direct specific transcription in tissues and testicular germ cell." in: The Biochemical journal, Vol. 335 ( Pt 2), pp. 259-65, 1998 (PubMed).

Lin, Huang, Liao et al.: "ARL4, an ARF-like protein that is developmentally regulated and localized to nuclei and nucleoli." in: The Journal of biological chemistry, Vol. 275, Issue 48, pp. 37815-23, 2000 (PubMed).