Interferon Regulatory Factor 8 (IRF8) antibody

Antigen: Interferon Regulatory Factor 8 (IRF8)

Synonyms: ICSBP, IRF-8, ICSBP1, H-ICSBP, Myls, Icsbp1, AI893568, IRF8, zgc:92252, icsbp1, MGC80112, MGC140338, DKFZp469B0320

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184813

Additional Information

Alternative name: ICSBP1 / IRF8

Gene ID: 3394, 15900 (mouse)

Immunogen: C-QRSFFRENQQITV

Description: All names and symbols: ICSBP1, IRF8, interferon consensus sequence binding protein 1, IRF-8, H-ICSBP, interferon regulatory factor 8. Official Symbol: ICSBP1. RefSeq number(s): NP_002154. Gene ontology terms: RNA polymerase II transcription factor activity, enhancer binding, regulation of transcription, DNA-dependent, immune response, negative regulation of transcription from Pol II promoter, nucleus

Specificity: Human, Mouse, Rat 426 aa

Application Details

Application Notes: Western blot: Approx 45-50kDa band seen in K562 lysate (Predicted MW of 47kDa according to NP_002154). Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184813 staining (1µg/ml) of K562 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Weisz, Marx, Sharf et al.: "Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25589-96, 1993 (PubMed).