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NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) antibody
| Antigen | NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80) |
| Synonyms | HEC, HEC1, TID3, KNTC2, hsNDC80, Kntc2, AV139730, 2610020P18Rik, CG9938, DmelCG9938, dmNdc80, Hec1, ndc80, wu:fb52e02, zgc:111801, NDC80 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN184821 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | HEC1 |
| Gene ID | 10403 |
| Immunogen | C-YEKKATLIKSSEE |
| Format | Affinity-purified |
| Description | All names and symbols: HEC1, HEC, highly expressed in cancer, rich in leucine heptad repeats, HEC-1. Official Symbol: KNTC2. RefSeq number(s): NP_006092. Gene ontology terms: mitotic chromosome segregation, mitosis, centromere, nucleus |
| Specificity | Human 642 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 75-80kDa band observed in Hela lysate (predicted MW of 74kDa according to NP_006092). Recommended for use at 0.5-2µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Cancer, Cell Cycle |
| Restrictions | For Research Use only |
Images
| ABIN184821 staining (0.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Chen, Riley, Chen et al.: "HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis." in: Molecular and cellular biology, Vol. 17, Issue 10, pp. 6049-56, 1997 (PubMed).
|
Alternatives
Alternatives for antigen "NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae) (NDC80)", type "Antibodies"
| Hosts | Rabbit (28), Mouse (5), Goat (4) |
| Reactivities | Human (36), Mouse (Murine) (6), Rat (Rattus) (5), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1), Pig (Porcine) (1) |
| Applications | Flow Cytometry (FACS) (20), Immunofluorescence (IF) (19), Western Blotting (WB) (18), ELISA (5), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Dot Blot (Dot) (1), Immunocytochemistry (ICC) (1), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | C-Term (2) |
