Dynactin antibody

Antigen: Dynactin

Synonyms: Chip150, DCTN1, MGC148413, DKFZp459A1630

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184834

Additional Information

Gene ID: 1639, 13191 (mouse)

Immunogen: C-QEQLHQLHSRLIS

Format: Affinity-purified

Description: All names and symbols: DCTN1, dynactin 1 (p150, glued homolog, Drosophila), P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog). Official Symbol: DCTN1. RefSeq number(s): NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1. Gene ontology terms: mitosis, cytoplasm, neurogenesis

Specificity: This antibody is expected to recognise all reported isoforms (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1). Human, Mouse 1278 aa, 1144 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 150kDa band observed in cell lline A549 and Human Testis lysates (calculated MW of 142kDa according to NP_004073.2). Recommended for use at 0.5-2µg/ml. Immunohistochemistry: In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. In paraffin embedded Human Testis shows cytoplasm staining in cells of the seminiferous tubules. Recommended concentration, 2-4µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184834 staining (1µg/ml) of Human Testis lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184834 (2.5µg/ml) staining of A) paraffin embedded Human Cerebellum and B) paraffin embedded Human Cerebellum. A) & B) Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Holzbaur, Tokito: "Localization of the DCTN1 gene encoding p150Glued to human chromosome 2p13 by fluorescence in situ hybridization." in: Genomics, Vol. 31, Issue 3, pp. 398-9, 1997 (PubMed).