MAX Dimerization Protein 4 (MXD4) antibody

Antigen: MAX Dimerization Protein 4 (MXD4)

Synonyms: MAD4, MST149, MSTP149, bHLHc12, xmad4, mst149, bhlhc12, mstp149, MGC84841, MXD4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184842

Additional Information

Alternative name: MAD4 / MXD4

Gene ID: 10608

Immunogen: GPHCRRLGRPALS

Format: Affinity-purified

Description: All names and symbols: MAD4, MXD4, MAX dimerization protein 4, Mad4 homolog, MST149, MSTP149. Official Symbol: MXD4. RefSeq number(s): NP_006445.1. Gene ontology terms: protein binding, transcription co-repressor, cell growth and maintenance, negative control of cell proliferation, repression of transcription from Pol II promoter

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 22kDa band observed in human kidney lysates (predicted MW of 24kDa according to NP_006445). Recommended for use at 0.5-2µg/ml. Please note the appearance of this band was blocked by incubation with the immunising peptide.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184842 staining (0.5µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hurlin, Queva, Koskinen et al.: "Mad3 and Mad4: novel Max-interacting transcriptional repressors that suppress c-myc dependent transformation and are expressed during neural and epidermal differentiation." in: The EMBO journal, Vol. 14, Issue 22, pp. 5646-59, 1996 (PubMed).