POU Domain Class 2-Associating Factor 1 (POU2AF1) antibody

Antigen: POU Domain Class 2-Associating Factor 1 (POU2AF1)

Synonyms: BOB1, OBF1, OCAB, OBF-1

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine)

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184851

Additional Information

Alternative name: BOB1 / OCAB / OBF1

Gene ID: 5450, 18985 (mouse)

Immunogen: C-DAYALNHTLSVEGF

Format: Affinity-purified

Description: All names and symbols: BOB1, OBF1, POU2AF1, OBF-1, POU domain, class 2, associating factor 1. Official Symbol: POU2AF1. RefSeq number(s): NP_006226. Gene ontology terms: transcription co-activator activity, cell growth and/or maintenance, humoral immune response, regulation of transcription, DNA-dependent, transcription from Pol II promoter, nucleus

Specificity: Human, Mouse, Rat 256 aa

Application Details

Application Notes: Western blot: Approx 30kDa band seen in mouse spleen lysate (Predicted MW of 28kDa according to NP_006226). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184851 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Strubin, Newell, Matthias: "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins." in: Cell, Vol. 80, Issue 3, pp. 497-506, 1995 (PubMed).