Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1) antibody

Antigen: Squamous Cell Carcinoma Antigen Recognized By T Cells (SART1)

Synonyms: Ara1, HOMS1, Snu66, MGC2038, SART1259, SNRNP110, U5-110K, DmelCG6686, SART1, MGC53679, MGC132129, zgc:91927

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184888

Additional Information

Alternative name: SART1

Gene ID: 9092, 20227 (mouse), 29678 (rat)

Immunogen: GSSKKHRGEKEAA-C

Format: Affinity-purified

Description: All names and symbols: SART1, squamous cell carcinoma antigen recognised by T cells, ARA1, Ara1, HOMS1, MGC2038, SART1259, Snu66, IgE autoantigen, SART1(259) protein, SART1(800) protein, U4/U6.U5 tri-snRNP-associated 110 kDa protein, squamous cell carcinoma antigen recognised by T cells, squamous cell carcinoma antigen recognized by T cells 1. Official Symbol: SART1. RefSeq number(s): NP_005137.1. Gene ontology terms: cytosol, cytoplasm, tumor antigen

Specificity: This antibody is expected to recognise both the human SART1(259) and SART1(800) proteins. Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Cell Cycle

Restrictions: For Research Use only

Images

ABIN184888 (0.03µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Vertegaal, Ogg, Jaffray et al.: "A proteomic study of SUMO-2 target proteins." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33791-8, 2004 (PubMed).