Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3) antibody

Antigen: Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3)

Synonyms: P100, p110, DSAP1, TIP110, KIAA0156, MGC138188, p110(nrb), RP11-13G14, SART3, MGC152480, DKFZp469J2418

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184889

Additional Information

Alternative name: SART3

Gene ID: 9733

Immunogen: C-MSNADFAKLFLRK

Format: Affinity-purified

Description: All names and symbols: SART3, squamous cell carcinoma antigen recognised by T cells 3, TIP110, KIAA0156, p110(nrb), RP11-13G14, tat-interacting protein of 110 kDa. Official Symbol: SART3. RefSeq number(s): NP_055521.1

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Approx 170kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 111kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yang et al, Cancer Res. 1999 Aug 15,59(16):4056-63, PMID: 10463607). Recommended concentration: 0.01-0.03µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer, Chromatin Binding Proteins

Restrictions: For Research Use only

Images

ABIN184889 staining (0.01µg/ml) of Jurkat cell lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Bell, Schreiner, Damianov et al.: "p110, a novel human U6 snRNP protein and U4/U6 snRNP recycling factor." in: The EMBO journal, Vol. 21, Issue 11, pp. 2724-35, 2002 (PubMed).