Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t)

Synonyms: PIG50, HSPC150, hspc150, MGC53460, C80607, 2700084L22Rik, RGD1310816, UBE2T, MGC133853

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184897

Additional Information

Alternative name: HSPC150 / UBE2T

Gene ID: 29089

Immunogen: Peptide with sequence C-QLVGIEKKFHPDV, from the C Terminus of the protein sequence according to NP_054895.1

Format: Affinity-purified

Description: All names and symbols: UBE2T, HSPC150, PIG50, HSPC150 protein similar to ubiquitin-conjugating enzyme, ubiquitin conjugating enzyme, ubiquitin-conjugating enzyme E2T (putative), ubiquitin-conjugating enzyme E2T. Official Symbol: UBE2T. RefSeq number(s): NP_054895.1. Gene ontology terms: ubiquitin conjugating enzyme activity, ubiquitin-protein ligase activity, ubiquitin cycle, protein modification, ligase activity.

Specificity: Expected from sequence similarity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16000. Western Blot: Approx. 22kDa band observed in lysates of cell lines K562, HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Recommended concentration: 0.1-0.3µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).