Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2F (Putative) (UBE2F)

Synonyms: AI851109, 2510010F15Rik, NCE2, MGC18120, MGC105540, RGD1307608, ube2f, MGC78786

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Mouse (Murine)

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184898

Additional Information

Alternative name: UBE2F/NEDD8-conjugating enzyme

Gene ID: 140739

Immunogen: C-RNKVDDYIKRYAR

Description: All names and symbols: UBE2F, ubiquitin-conjugating enzyme E2F (putative), MGC18120, NCE2, NEDD8 conjugating enzyme, NEDD8-conjugating enzyme. Official Symbol: UBE2F. RefSeq number(s): NP_542409. Gene ontology terms: ligase activity, ubiquitin conjugating enzyme activity, ubiquitin cycle

Specificity: Human 185 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Kidney and Liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any res EB05766 has been succesfully used in WB assays using Human Cell lysate (whole cell lines of HeLa, Jurkat, U2OS, 293T and IRM9, loaded 20ug, 5% milk as blocking agent for 12 hours at 4°C, primary incubation 2 hours with TBS-T with a dilution of 1:1000 ) (

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Podust, Brownell, Gladysheva et al.: "A Nedd8 conjugation pathway is essential for proteolytic targeting of p27Kip1 by ubiquitination." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 9, pp. 4579-84, 2000 (PubMed).