Midline 2 (MID2) antibody

Antigen: Midline 2 (MID2)

Synonyms: FXY2, RNF60, TRIM1, FLJ37715, FLJ41813, Trim1, RGD1565674, MID2, fxy2, rnf60, trim1

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184920

Additional Information

Alternative name: MID2 / TRIM1

Gene ID: 11043, 23947 (mouse)

Immunogen: QESPYVSGMKTCH

Format: Affinity-purified

Description: All names and symbols: MID2, TRIM1, midline 2, FXY2, midin 2, tripartite motif protein 1. Official Symbol: MID2. RefSeq number(s): NP_036348.1, NP_438112.1. Gene ontology terms: microtubule associated protein, embryogenesis and morphogenesis, cell shape and cell size control

Specificity: This antibody is expected to recognise both human isoforms of this protein. Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 80kDa band observed in mouse heart lysates (calculated MW of 81.3kDa according to NP_036348.1 and 77.9kDa according to NP_438112.1). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining in cytoplasm and brush border of some epithelial cells of proximal convoluted tubules. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184920 (1µg/ml) staining of mouse heart lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184920 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Buchner, Montini, Andolfi et al.: "MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development." in: Human molecular genetics, Vol. 8, Issue 8, pp. 1397-407, 1999 (PubMed).