Polycomb Group Ring Finger 3 (PCGF3) (AA 247 isoform) antibody
| Antigen | Polycomb Group Ring Finger 3 (PCGF3) |
| Synonyms | RNF3, DONG1, RNF3A, FLJ36550, FLJ43813, MGC40413, MGC129615, DKFZp686D20235, Rnf3, AI662857, E430039C14, 2310035N15Rik, D630042K08Rik, rnf3, dong1, rnf3a, PCGF3, MGC129075 |
| Binding Site |
 Alternatives AA 247 isoform |
| Clonality | Polyclonal |
| Host |
Alternatives Goat |
| Reactivity |
Alternatives Human |
| Conjugate |
Alternatives Un-conjugated |
| Application |
Alternatives ELISA, Western Blotting (WB) |
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1 reference available |
| Catalog no. | ABIN184926 |
| Quantity | 100 ug |
| Price | 374.27 $ Plus shipping costs $45.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | RNF3 |
| Gene ID | 10336, 69587 (mouse) |
| Immunogen | C-PLLLHYRPKMDLL |
| Description | All names and symbols: RNF3, DONG1, RNF3A, MGC40413, ring finger protein 3, PCGF3 , polycomb group ring finger 3. Official Symbol: PCGF3. RefSeq number(s): NP_006306 |
| Specificity | This antibody is expected to recognise the 247 aa isoform only (NP_006306), not the 132 aa isoform (NP_694982). Human, Mouse 247 aa, 132 aa |
Application Details
| Application Notes | Peptide ELISA: Pass 1/2000. Western Blot: Approx 35kDa band seen in Human Heart lysate [Predicted MW of approx. 27kDa according to NP_006306]. Recommended for use at 0.5-2µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Proteolysis / Ubiquitin |
| Restrictions | For Research Use only |
Images
| ABIN184926 staining (1µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
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Freemont: "The RING finger. A novel protein sequence motif related to the zinc finger." in: Annals of the New York Academy of Sciences, Vol. 684, pp. 174-92, 1993 (PubMed).
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Alternatives
Alternatives for antigen "Polycomb Group Ring Finger 3 (PCGF3)", type "Antibodies"
| Hosts | Rabbit (22), Goat (3), Mouse (1) |
| Reactivities | Human (24), Cow (Bovine) (21), Mouse (Murine) (20), Rat (Rattus) (20) |
| Applications | Immunofluorescence (IF) (15), Western Blotting (WB) (9), ELISA (5), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunoelectron Microscopy (IEM) (1) |
| Conjugates | Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1) |
| Epitopes | Internal Region (2), C-Term (1), C-Term,AA 247 (1) |
Sponsorship
2013 BioMed Central Research Awards
2013 BioMed Central Research Awards
