STE20-Related Kinase Adaptor beta (STRADB) antibody

Antigen: STE20-Related Kinase Adaptor beta (STRADB)

Synonyms: PAPK, ILPIP, ILPIPA, ALS2CR2, CALS-21, PRO1038, MGC102916

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184938

Additional Information

Alternative name: ALS2CR2 / ILPIP

Gene ID: 55437

Immunogen: Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence according to NP_061041.2, AAF71042.1

Format: Affinity-purified

Description: All names and symbols: STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2. Official Symbol: STRADB. RefSeq number(s): NP_061041.2, AAF71042.1. Gene ontology terms: ATP binding, protein kinase activity, protein amino acid phosphorylation, cellular_component unknown, transferase activity This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1)..

Specificity: Expected from sequence similarity: Human, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1(beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration 1-3µg/ml. Immunohistochemistry: Immunohistochemistry: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration, 3-5µg/ml.

Concentration: 100µg specific antibody in 200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: For long-term storage keep aliquots at -20°C. (Store no longer than 12 months at 4°C). Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Publications: Sanna, da Silva Correia, Luo et al.: "ILPIP, a novel anti-apoptotic protein that enhances XIAP-mediated activation of JNK1 and protection against apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30454-62, 2002 (PubMed).