Tripartite Motif Containing 63 (TRIM63) (N-Term) antibody

Antigen: Tripartite Motif Containing 63 (TRIM63)

Synonyms: IRF, SMRZ, MURF1, MURF2, RNF28, FLJ32380, Murf, Murf1, Rnf28, RF1, MuRF1, rnf30, MGC80210, TRIM63, MGC137423, MuRF

Epitope: N-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184950

Additional Information

Alternative name: MURF1 / TRIM63 (N Term)

Gene ID: 84676

Immunogen: DYKSSLIQDGNPM-C

Format: Affinity-purified

Description: All names and symbols: MURF1, MURF-1, RNF28, ring finger protein 28, IRF, SMRZ, MURF2, FLJ32380, iris ring finger protein, muscle specific ring finger protein 2, striated muscle RING zinc finger protein. Official Symbol: TRIM63. RefSeq number(s): NP_115977.2. Gene ontology terms: zinc ion binding, signal transducer activity, signal transduction, kinesin complex, microtubule, cytoplasm, nucleus

Specificity: Human, Dog 353 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Skeletal Muscle lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_115977.2. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Skeletal Muscle shows strong staining in rare nuclei, and weak staining of muscle fibres in longitudinal section.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184950 (1.25µg/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Dai, Liew: "A novel human striated muscle RING zinc finger protein, SMRZ, interacts with SMT3b via its RING domain." in: The Journal of biological chemistry, Vol. 276, Issue 26, pp. 23992-9, 2001 (PubMed).