Parkinson Protein 7 (PARK7) antibody

Antigen: Parkinson Protein 7 (PARK7)

Synonyms: DJ1, DJ-1, FLJ27376, FLJ34360, FLJ92274

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184951

Additional Information

Alternative name: PARK7 / DJ-1

Gene ID: 11315, 57320 (mouse), 117287 (rat)

Immunogen: C-AAQVKAPLVLKD

Format: Affinity-purified

Description: All names and symbols: PARK7, DJ1, DJ-1, Parkinson disease (autosomal recessive, early onset) 7, oncogene DJ1. Official Symbol: PARK7. RefSeq number(s): NP_009193. Gene ontology terms: RAS protein signal transduction, cytoplasm, nucleus

Specificity: Human, Mouse, Rat 189 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 20kDa band seen in Jurkat lysate [Predicted MW of approx. 20kDa according to NP_009193]. Recommended for use at 0.5-1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184951 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Nagakubo, Taira, Kitaura et al.: "DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras." in: Biochemical and biophysical research communications, Vol. 231, Issue 2, pp. 509-13, 1997 (PubMed).