Tripartite Motif Containing 40 (TRIM40) antibody

Antigen: Tripartite Motif Containing 40 (TRIM40)

Synonyms: RNF35, Gm35, Gm319, Gm865, Rnf35, MGC166221

Clonality: Polyclonal

Host: Goat

Reactivity: Please enquire

Application: ELISA

Catalog no.: ABIN184959

Additional Information

Alternative name: RNF35 / TRIM40

Gene ID: 135644

Immunogen: C-GVSELLLQPPQKL

Description: All names and symbols: TRIM40, tripartite motif-containing 40, RNF35, ring finger RNF35. Official Symbol: TRIM40. RefSeq number(s): NP_619645. Gene ontology terms: zinc ion binding, intracellular

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32000. Western Blot: Approx. 45kDa band observed in Human Brain lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 26kDa according to NP_619645. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Freemont: "The RING finger. A novel protein sequence motif related to the zinc finger." in: Annals of the New York Academy of Sciences, Vol. 684, pp. 174-92, 1993 (PubMed).