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Sorting Nexin 26 (SNX26) (C-Term) antibody

Details for Product No. ABIN184975
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Antigen
Synonyms SNX26, tcgap, NOMA-GAP, TCGAP, AW324378, Snx26
Epitope
C-Term
(6), (5), (1)
Reactivity
Human
(14), (2)
Host
Goat
(12), (2)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(10), (8), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184975
374.27 $
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Immunogen Peptide with sequence SWSLHSEGQTRSYC, from the C Terminus of the protein sequence according to NP_443180.2.
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SNX26 / TCGAP
Background Synonyms: SNX26, TCGAP, FLJ39019, sorting nexin 26, likely ortholog of mouse TC10/CDC42 GTPase-activating protein, NOMA-GAP, neurite outgrowth multiadaptor RhoGAP protein
Gene ID 115703, 233071
NCBI Accession NP_443180.2
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Preliminary experiments gave an approx 75 kDa band in Human Bone Marrow, Kidney, and Pancreas lysates after 1 µg/mL antibody staining. EB05916 may detect the isoform A1A5D2 (UniProtKB) with a molecular weight of 81.4 kDa which is similar in amino acid sequence to NP_443180.2 however lacking aa729-1268. Please note that currently we cannot find a precise explanation in the literature for the band we observe given the calculated size of 121 kDa according to NP_443180.2. The 75 kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Brain shows cytoplasm staining of Purkinje cells in Cerebellum. Recommended concentration: 2.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Sorting Nexin 26 (SNX26) (C-Term) antibody ABIN184975 (2.5µg/ml) staining of paraffin embedded Human Brain. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Teasdale, Loci, Houghton et al.: "A large family of endosome-localized proteins related to sorting nexin 1." in: The Biochemical journal, Vol. 358, Issue Pt 1, pp. 7-16, 2001 (PubMed).

Validation Images
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