Polycomb Group Ring Finger 2 (PCGF2) antibody

Antigen: Polycomb Group Ring Finger 2 (PCGF2)

Synonyms: MEL-18, RNF110, ZNF144, MGC10545, Mel18, Rnf110, Zfp144, mel-18, PCGF2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184999

Additional Information

Alternative name: MEL18 / PCGF2

Gene ID: 7703

Immunogen: C-KMTVNGAPVPPLT

Format: Affinity-purified

Description: All names and symbols: PCGF2, MEL-18, polycomb group ring finger 2, RNF110, ZNF144, ring finger protein 110, zinc finger protein 144. Official Symbol: PCGF2. RefSeq number(s): NP_009075. Gene ontology terms: transcription factor activity, regulation of transcription, DNA-dependent, nucleus

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 40 kDa band observed in Human Lung extracts (predicted MW of 40kDa according to NP_009075). Recommended for use at 1-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184999 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Ishida, Asano, Hasegawa et al.: "Cloning and chromosome mapping of the human Mel-18 gene which encodes a DNA-binding protein with a new 'RING-finger' motif." in: Gene, Vol. 129, Issue 2, pp. 249-55, 1993 (PubMed).