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Pescadillo Homolog 1, Containing BRCT Domain (Zebrafish) (PES1) antibody

Antigen

Pescadillo Homolog 1, Containing BRCT Domain (Zebrafish) (PES1)

Synonyms PES, pes1, MGC53969, GB15362, pesc
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Application
Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN185017
Quantity 100µg  (100µg/200µl)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name Pescadillo
Gene ID 23481, 64934 (mouse)
Swiss-Prot NP_055118.1
Immunogen Peptide with sequence GGLEKKKYERGSA-C, from the N Terminus of the protein sequence according to NP_055118.1.
Cross-Reactivity Human, Mouse (Murine), Pig (Porcine), Cow (Bovine)
Format Liquid
Grade Serum-free
Description Official Symbol: PES1
Specificity Human, Mouse 588 aa

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 70kDa band observed in HeLa lysate (calculated MW of 68kDa according to NP_055118). Recommended concentration: 1-3µg/ml. IHC: In paraffin embedded Human Testis shows nucleolar staining in spermatocytes and spermatids. Recommended concentration, 2.5-3.8µg/ml.
Concentration 100µg/200µl
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Research Area Cancer, Heat Shock Proteins
Restrictions For Research Use only

Publications

Publications Allende, Amsterdam, Becker et al.: "Insertional mutagenesis in zebrafish identifies two novel genes, pescadillo and dead eye, essential for embryonic development." in: Genes & development, Vol. 10, Issue 24, pp. 3141-55, 1997 (PubMed).

Alternatives

Alternatives for antigen "Pescadillo Homolog 1, Containing BRCT Domain (Zebrafish) (PES1)", type "Antibodies"
Hosts Rabbit (20), Goat (3), Mouse (3)
Reactivities Human (25), Mouse (Murine) (21), Rat (Rattus) (20), Cow (Bovine) (17), Pig (Porcine) (17), Dog (Canine) (16), Horse (Equine) (16), Rabbit (16), Chicken (1)
Applications Immunofluorescence (IF) (20), Flow Cytometry (FACS) (18), Western Blotting (WB) (8), ELISA (Detection) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes N-Term (1)