Ubiquitin Specific Peptidase 20 (USP20) antibody

Antigen: Ubiquitin Specific Peptidase 20 (USP20)

Synonyms: USP20, usp20, VDU2, LSFR3A, KIAA1003, Vdu2, AI467231, 1700055M05Rik, MGC63661, zgc:63661, im:7151955, vdu2, lsfr3a, DKFZp459P1127

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185019

Additional Information

Alternative name: USP20 / VDU2

Gene ID: 10868, 74270 (mouse)

Immunogen: C-HGEQKIEAETRAV

Format: Affinity-purified

Description: All names and symbols: USP20, ubiquitin specific protease 20, VDU2, KIAA1003, pVHL-interacting deubiquitinating enzyme 2. Official Symbol: USP20. RefSeq number(s): NP_006667. Gene ontology terms: cysteine-type endopeptidase activity, ubiquitin-dependent protein catabolism, ubiquitin thiolesterase activity, cellular_component unknown, hydrolase activity, protein deubiquitination

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave no signal but low background in Human Liver extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been Immunohistochemistry: In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Recommended concentration, 3 µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185019 (3 ug/ml) staining of paraffin embedded Human Lymph Node. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Curcio-Morelli, Zavacki, Christofollete et al.: "Deubiquitination of type 2 iodothyronine deiodinase by von Hippel-Lindau protein-interacting deubiquitinating enzymes regulates thyroid hormone activation." in: The Journal of clinical investigation, Vol. 112, Issue 2, pp. 189-96, 2003 (PubMed).