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FOXC1 antibody (Forkhead Box C1) (C-Term)

Details for Product anti-FOXC1 Antibody No. ABIN185028, Supplier: Log in to see
Antigen
  • ara
  • fkhl7
  • freac-3
  • freac3
  • igda
  • ihg1
  • irid1
  • rieg3
  • xfd-11
  • FOXC1
  • foxc1
  • ARA
  • FKHL7
  • FREAC-3
  • FREAC3
  • IGDA
  • IHG1
  • IRID1
  • RIEG3
  • Fkh1
  • Mf1
  • Mf4
  • ch
  • fkh-1
  • frkhda
  • CFKH-1
Epitope
C-Term
41
13
7
5
4
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Zebrafish
81
28
7
4
3
3
2
2
1
1
Host
Goat
50
34
5
Clonality
Polyclonal
Conjugate
This FOXC1 antibody is un-conjugated
6
5
4
4
4
4
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
77
54
18
13
10
4
4
3
1
1
1
1
Supplier
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Immunogen Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.
Blocking Peptide Blocking peptide for this product available: ABIN370432
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXC1 (FOXC1 Antibody Abstract)
Background Synonyms: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IRID1, FREAC3, iridogoniodysgenesis type 1, forkhead (Drosophila)-like 7, forkhead-related activator 3, Forkhead, drosophila, homolog-like 7, FREAC-3, RIEG3, forkhead/winged helix-like transcription factor 7, myeloid factor-delta
Gene ID 2296, 17300
NCBI Accession NP_001444
Pathways
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: In transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1 µg/mL. An anonymous customer found positive results in WB on Human breast cancer cells (T47D).
IHC: In paraffin embedded Human Kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. Recommended concentration: 4-6 µg/mL. Customer successfully used in IHC-P in mouse (periocular mesenchyme, cornea) Chromatin immunoprecipitation: Customer successfully used this antibody in ChIP from human cell line. This antibody has also been successfully described in a paper on zebrafish embryos (PubMed: 17000708).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-FOXC1 antibody (Forkhead Box C1) (C-Term) (ABIN185028) ABIN185028 staining (0.5µg/ml) of Human Bone Marrow lysate (RIPA buffer, 35µg total p...
Immunohistochemistry (IHC) image for anti-FOXC1 antibody (Forkhead Box C1) (C-Term) (ABIN185028) ABIN185028 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen re...
Background publications Zhou, Kato, Asanoma et al.: "Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth." in: Genomics, Vol. 80, Issue 5, pp. 465-72, 2002 (PubMed).