Forkhead Box C1 (FOXC1) (C-Term) antibody

Details for Product No. ABIN185028
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Antigen
Synonyms ara, fkhl7, freac-3, freac3, igda, ihg1, irid1, rieg3, xfd-11, FOXC1, foxc1, ARA, FKHL7, FREAC-3, FREAC3, IGDA, IHG1, IRID1, RIEG3, Fkh1, Mf1, Mf4, ch, fkh-1, frkhda, CFKH-1
Epitope
C-Term
(18), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine), Zebrafish
(54), (29), (16), (14), (12), (12), (3), (3), (2), (1), (1)
Host
Goat
(42), (13), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(46), (23), (16), (10), (5), (4), (4), (3), (2), (2), (2), (2), (1)
Pubmed 1 reference available
Quantity 100 μg
Options
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Catalog No. ABIN185028
374.27 $
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  • +1 404 474 4654
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Immunogen Peptide with sequence RTSGAFVYDCSKF, from the C Terminus of the protein sequence according to NP_001444.2.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXC1
Background Synonyms: FOXC1, forkhead box C1, ARA, IGDA, IHG1, FKHL7, IRID1, FREAC3, iridogoniodysgenesis type 1, forkhead (Drosophila)-like 7, forkhead-related activator 3, Forkhead, drosophila, homolog-like 7, FREAC-3, RIEG3, forkhead/winged helix-like transcription factor 7, myeloid factor-delta
Gene ID 2296, 17300
NCBI Accession NP_001444
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: In transfected HEK293 transiently expressing full-length Human FOXC1 (myc and DYKDDDDK tagged), a band of approx. 75 kDa was observed. No bands were observed in mock-transfected and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration: 0.5-1 µg/mL. An anonymous customer found positive results in WB on Human breast cancer cells (T47D).
IHC: In paraffin embedded Human Kidney shows nuclear staining in DCT and select nuclear staining in the glomerulus. Recommended concentration: 4-6 µg/mL. Customer successfully used in IHC-P in mouse (periocular mesenchyme, cornea) Chromatin immunoprecipitation: Customer successfully used this antibody in ChIP from human cell line. This antibody has also been successfully described in a paper on zebrafish embryos (PubMed: 17000708).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Forkhead Box C1 (FOXC1) (C-Term) antibody ABIN185028 staining (0.5µg/ml) of Human Bone Marrow lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
anti-Forkhead Box C1 (FOXC1) (C-Term) antibody (2) ABIN185028 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
General Zhou, Kato, Asanoma et al.: "Identification of FOXC1 as a TGF-beta1 responsive gene and its involvement in negative regulation of cell growth." in: Genomics, Vol. 80, Issue 5, pp. 465-72, 2002 (PubMed).

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