F-Box and WD Repeat Domain Containing 2 (FBXW2) antibody

Antigen: F-Box and WD Repeat Domain Containing 2 (FBXW2)

Synonyms: Md6, FBW2, Fwd2, MGC117371, MD6, 2700071L08Rik, fbxw2, MGC53244, FBXW2, md6, fbw2, fwd2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185067

Additional Information

Alternative name: FBXW2

Gene ID: 26190

Immunogen: C-KRGSSFLAGEHPG

Format: Affinity-purified

Description: All names and symbols: FBXW2, F-box and WD-40 domain protein 2, FBW2, Fwd2, MGC117371, Md6, F-box protein Fbw2. Official Symbol: FBXW2. RefSeq number(s): NP_036296.1. Gene ontology terms: ubiquitin conjugating enzyme activity, ubiquitin-protein ligase activity, proteolysis and peptidolysis, protein modification

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 48kDa band observed in Human Kidney lysates (calculated MW of 48.0kDa according to NP_036296.1). Recommended concentration: 0.3-1.5µg/ml. A stronger band of unknown identity was also consistently observed at appr. 30kDa - both bands were successfully blocked by incubation with the immunising peptide. Immunohistochemistry: In paraffin embedded Human Kidney, shows cytoplasmic staining of epithelial cells in some renal tubules. Recommended concentration, 2-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185067 staining (0.3µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN185067 (3µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

Publications

Publications: Winston, Koepp, Zhu et al.: "A family of mammalian F-box proteins." in: Current biology : CB, Vol. 9, Issue 20, pp. 1180-2, 1999 (PubMed).