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Anillin, Actin Binding Protein (ANLN) (C-Term) antibody

Antigen

Anillin, Actin Binding Protein (ANLN)
Synonyms scra, Scraps, DKFZp779A055, RGD1566097, ANLN, C78101, 1110037A17Rik, 2900037I21Rik, scraps, ABP8, Ani, Scra, anillin, l(2)03427, l(2)43Ec, DmelCG2092, CG2092
Epitope
Alternatives

C-Term
Clonality Polyclonal
Host
Reactivity
Alternatives

Human
Application
Alternatives ELISA, Immunohistochemistry (IHC)
1 reference available
Catalog no. ABIN185086
Quantity 100 ug  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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anti-Anillin, Actin Binding Protein (ANLN) (C-Term) antibody

Additional Information
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Alternative name ANILLIN / Scraps (C Terminus)
Gene ID 54443, 68743 (mouse)
Immunogen WQPDACYKPIGKP
Format Affinity-purified
Description All names and symbols: Scraps, ANILLIN, ANLN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein. Official Symbol: ANLN. RefSeq number(s): NP_061155.2. Gene ontology terms: actin binding, regulation of exit from mitosis, septin assembly and septum formation
Specificity Human, Mouse

Application Details
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Application Notes Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications
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Publications Oegema, Savoian, Mitchison et al.: "Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis." in: The Journal of cell biology, Vol. 150, Issue 3, pp. 539-52, 2000 (PubMed).

Alternatives
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Alternatives for antigen "Anillin, Actin Binding Protein (ANLN)", type "Antibodies"
Hosts Goat (8), Mouse (1)
Reactivities Human (8), Mouse (Murine) (7), Cow (Bovine) (2), Dog (Canine) (2), Rat (Rattus) (2)
Applications Western Blotting (WB) (5), Enzyme Immunoassay (EIA) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), ELISA (1), ELISA (Detection) (1), Immunofluorescence (IF) (1)
Epitopes C-Term (3), internal (3)