Anillin, Actin Binding Protein (ANLN) (C-Term) antibody

Antigen: Anillin, Actin Binding Protein (ANLN)

Synonyms: scra, Scraps, DKFZp779A055, RGD1566097, ANLN, C78101, 1110037A17Rik, 2900037I21Rik, scraps, ABP8, Ani, Scra, anillin, l(2)03427, l(2)43Ec, DmelCG2092, CG2092

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185086

Additional Information

Alternative name: ANILLIN / Scraps (C Terminus)

Gene ID: 54443, 68743 (mouse)

Immunogen: WQPDACYKPIGKP

Format: Affinity-purified

Description: All names and symbols: Scraps, ANILLIN, ANLN, anillin, actin binding protein (scraps homolog, Drosophila), anillin (Drosophila Scraps homolog), actin binding protein. Official Symbol: ANLN. RefSeq number(s): NP_061155.2. Gene ontology terms: actin binding, regulation of exit from mitosis, septin assembly and septum formation

Specificity: Human, Mouse

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 75kDa band in Mouse Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 124kDa according to NP_061155.2. The 75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Kidney shows staining of nuclei in some cells of renal tubules.. Recommended concentration, 3-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185086 (10µg/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Oegema, Savoian, Mitchison et al.: "Functional analysis of a human homologue of the Drosophila actin binding protein anillin suggests a role in cytokinesis." in: The Journal of cell biology, Vol. 150, Issue 3, pp. 539-52, 2000 (PubMed).