ArfGAP with Dual PH Domains 2 (ADAP2) antibody

Antigen: ArfGAP with Dual PH Domains 2 (ADAP2)

Synonyms: CENTA2, cent-b, HSA272195, Centa2

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185095

Additional Information

Alternative name: CENTA2

Gene ID: 55803

Immunogen: C-RLTASTESGRSSR

Format: Affinity-purified

Description: All names and symbols: CENTA2, centaurin, alpha 2, HSA272195, centaurin-alpha 2 protein. Official Symbol: CENTA2. RefSeq number(s): NP_060874

Specificity: Human 381 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: preliminary experiments in Human Brain and Heart lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Placenta shows trong staining of cytoplasm in syncytiotrophoblasts.. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185095 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Jenne, Tinschert, Stegmann et al.: "A common set of at least 11 functional genes is lost in the majority of NF1 patients with gross deletions." in: Genomics, Vol. 66, Issue 1, pp. 93-7, 2000 (PubMed).