ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2) antibody

Antigen: ArfGAP with Coiled-Coil, Ankyrin Repeat and PH Domains 2 (ACAP2)

Synonyms: CENTB2, CNT-B2, KIAA0041, Centb2, mKIAA0041, 4832442G16, 9530039J15Rik, centb2, MGC81056, ACAP2, MGC139548, cenB1A, acap2, centb5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN185097

Additional Information

Alternative name: CENTB2 / ACAP2

Gene ID: 23527, 78618 (mouse)

Immunogen: C-EKLNRFQQDSQKF

Format: Affinity-purified

Description: All names and symbols: CENTB2, ACAP2, centaurin, beta 2, CNT-B2, KIAA0041, centaurin beta2, Arf GAP with coiled coil, ANK repeat and PH domains 2. Official Symbol: CENTB2. RefSeq number(s): NP_036419

Specificity: Human, Mouse 778 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 90kDa band observed in Human T cell line MOLT4 lysates (calculated MW of 88.1kDa according to NP_036419). Recommended concentration: 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Lung shows strong staining of Type II pneumocytes. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185097 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

ABIN185097 (5µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Jackson, Brown, Nie et al.: "ACAPs are arf6 GTPase-activating proteins that function in the cell periphery." in: The Journal of cell biology, Vol. 151, Issue 3, pp. 627-38, 2000 (PubMed).