AGAP3 antibody (C-Term)

Catalog No. ABIN185100

The Goat Polyclonal anti-AGAP3 antibody (ABIN185100) specifically detects AGAP3 in ELISA and IHC. The antibody is reactive with Human samples.

Quick Overview for AGAP3 antibody (C-Term) (ABIN185100)

Target: AGAP3 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This AGAP3 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-AGAP3 Antibody

Binding Specificity: C-Term

Purpose: CENTG3 / MRIP1

Sequence: NPSAELHRSP SLL

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NPSAELHRSPSLL, from the C Terminus of the protein sequence according to NP_114152.3.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10 μg/mL.

Western Blot: Preliminary experiments gave an approx 45 kDa band in Human Brain and Human Liver lysates at 1 μg/mL. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in th

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for AGAP3

Target: AGAP3 (ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3))

Alternative Name: AGAP3

Background: AGAP3, CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein, ArfGAP with GTPase domain, ankyrin repeat and PH domain 3, CRAG, FLJ16146, FLJ34452, CRMP (collapsin response mediator protein) associated, centaurin, gamma 3

Gene ID: 116988, 213990

NCBI Accession: NP_114152

Pathways: Protein targeting to Nucleus