ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3) antibody

Antigen: ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 3 (AGAP3)

Synonyms: CRAG, CENTG3, MRIP-1, FLJ16146, FLJ34452, Crag, Centg3, AV028425, MGC37541, AGAP3

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185100

Additional Information

Alternative name: CENTG3 / MRIP1

Gene ID: 116988, 213990 (mouse)

Immunogen: C-NPSAELHRSPSLL

Description: All names and symbols: CENTG3, MRIP1, centaurin, gamma 3, MRIP-1, MRIP-1 protein. Official Symbol: CENTG3. RefSeq number(s): NP_114152. Gene ontology terms: GTP binding, regulation of GTPase activity, small GTPase mediated signal transduction

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain and Human Liver lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 101kDda according to NP_114152. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Kidney shows strong staining of brush borders in Proximal Convoluted tubules. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185100 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Surks, Richards, Mendelsohn: "Myosin phosphatase-Rho interacting protein. A new member of the myosin phosphatase complex that directly binds RhoA." in: The Journal of biological chemistry, Vol. 278, Issue 51, pp. 51484-93, 2003 (PubMed).