rho GTPase Activating Protein 26 (ARHGAP26) antibody

Antigen: rho GTPase Activating Protein 26 (ARHGAP26)

Synonyms: GRAF, OPHN1L, OPHN1L1, FLJ42530, KIAA0621, ARHGAP26, AI853435, mKIAA0621, 1810044B20Rik, 2610010G17Rik, 4933432P15Rik

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185109

Additional Information

Alternative name: GRAF / ARHGAP26

Gene ID: 23092, 71302 (mouse)

Immunogen: C-KTGLIPENYVEFL

Format: Affinity-purified

Description: All names and symbols: ARHGAP26, GRAF, Rho GTPase activating protein 26 , KIAA0621, FLJ42530, OPHN1L, OPHN1L1, GTPase regulator associated with FAK, GTPase regulator associated with focal adhesion kinase pp125(FAK), GTPase regulator associated with the focal adhesion kinase pp125. Official Symbol: ARHGAP26. RefSeq number(s): NP_055886.1. Gene ontology terms: Rho GTPase activator activity, actin cytoskeleton organization and biogenesis, cell growth and/or maintenance, neurogenesis,

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 92.2kDa according to NP_055886.1). Recommended concentration: 0.3-1µg/ml. An additional strong band of unknown identity was also consistently observed at 16kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185109 (0.3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Borkhardt, Bojesen, Haas et al.: "The human GRAF gene is fused to MLL in a unique t(5;11)(q31;q23) and both alleles are disrupted in three cases of myelodysplastic syndrome/acute myeloid leukemia with a deletion 5q." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 16, pp. 9168-73, 2000 (PubMed).