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Details for Product No. ABIN185112

Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody

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Antigen
Synonyms NET1A, ARHGEF8, Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik, arhgef8, net1a, xNET1, zgc:92121, wu:fb13g03, wu:fb25c11, DKFZp459I0423, NET1
Epitope
»Alternatives C-Term
Reactivity
»Alternatives Human
Host
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Methode Type Peptide ELISA
Application
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN185112
Quantity 100 µg
Price
374.27 $   Plus shipping costs $45.00
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Availability Ships within 5 Business Days
Immunogen Peptide with sequence C-DKALSGGKRKETLV, from the C Terminus of the protein sequence according to NP_001040625.1, NP_005854.2.
Specificity This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NET1 / ARHGEF8
Background Synonyms: NET1, ARHGEF8, NET1A, neuroepithelial cell transforming gene 1, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelial cell transforming 1, RP11-116G8.1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Gene ID 10276
NCBI Accession NP_001040625.1, NP_005854.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 85 kDa in Human Placenta and a very strong band at approx 40-50 kDa in Human Skeletal Muscle lysates after 0.1 µg/mL staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7 kDa according to NP_001040625.1 and of 61.9 kDa according to NP_005854.2.
IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration: 2.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Chan, Takai, Yamada et al.: "Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning." in: Oncogene, Vol. 12, Issue 6, pp. 1259-66, 1996 (PubMed).

Alternatives for antigen "Neuroepithelial Cell Transforming 1 (NET1)", type "Antibodies"
Hosts (58), (14), (5), (1)
Reactivities (72), (49), (44), (40), (37), (24), (24), (1), (1)
Applications (29), (26), (24), (23), (4), (3), (1), (1), (1), (1), (1)
Conjugates (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Epitopes (8), (5), (2), (2), (1), (1), (1), (1), (1)
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