Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody

Antigen: Neuroepithelial Cell Transforming 1 (NET1)

Synonyms: NET1A, ARHGEF8, Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik, MGC114559, MGC127767, net1a, xNET1, arhgef8, MGC53602, MGC145538, DKFZp459I0423, net1, NET1, zgc:92121, wu:fb13g03, wu:fb25c11

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185112

Additional Information

Alternative name: NET1 / ARHGEF8 (C Terminus)

Gene ID: 10276

Immunogen: C-DKALSGGKRKETLV

Format: Affinity-purified

Description: All names and symbols: NET1, ARHGEF8, NET1A, neuroepithelial cell transforming gene 1, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene). Official Symbol: NET1. RefSeq number(s): NP_001040625.1, NP_005854.2. Gene ontology terms: guanyl-nucleotide exchange factor activity, signal transduction

Specificity: This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2). Human, Mouse

Synonyms: NET1A, ARHGEF8, Net1a, mNET1, AI604373, AU015857, 0610025H04Rik, 9530071N24Rik, MGC114559, MGC127767, net1a, xNET1, arhgef8, MGC53602, MGC145538, DKFZp459I0423, net1, NET1, zgc:92121, wu:fb13g03, wu:fb25c11

Application Details

Application Notes: Preliminary experiments gave bands at approx 85kDa in Human Placenta and at very strong band at approx 40-50kDa in Human Skeletal Muscle extracts at 0.1µg/ml. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7kDa according to NP_001040625.1 and of 61.9kDa according to NP_005854.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration, 2.5µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185112 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Chan, Takai, Yamada et al.: "Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning." in: Oncogene, Vol. 12, Issue 6, pp. 1259-66, 1996 (PubMed).