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Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody

Details for Product No. ABIN185112, Supplier: Log in to see
Antigen
  • arhgef8
  • net1a
  • xNET1
  • DKFZp459I0423
  • NET1
  • ARHGEF8
  • NET1A
  • 0610025H04Rik
  • 9530071N24Rik
  • AI604373
  • AU015857
  • Net1a
  • mNET1
  • wu:fb13g03
  • wu:fb25c11
  • zgc:92121
Alternatives
anti-Human Neuroepithelial Cell Transforming 1 antibody for ELISA
Epitope
C-Term
25
15
13
10
3
3
2
2
2
1
1
1
Reactivity
Human
114
38
31
5
3
2
2
1
1
1
1
1
Host
Goat
81
26
6
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
7
3
3
3
3
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
54
50
30
17
14
3
2
2
1
Supplier
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Immunogen Peptide with sequence C-DKALSGGKRKETLV, from the C Terminus of the protein sequence according to NP_001040625.1, NP_005854.2.
Blocking Peptide Blocking peptide for this product available: ABIN368871
Specificity This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NET1 / ARHGEF8 (NET1 Antibody Abstract)
Background Synonyms: NET1, ARHGEF8, NET1A, neuroepithelial cell transforming gene 1, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelial cell transforming 1, RP11-116G8.1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Gene ID 10276
NCBI Accession NP_001040625, NP_005854
Pathways Neurotrophin Signaling Pathway
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 85 kDa in Human Placenta and a very strong band at approx 40-50 kDa in Human Skeletal Muscle lysates after 0.1 µg/mL staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7 kDa according to NP_001040625.1 and of 61.9 kDa according to NP_005854.2.
IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration: 2.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Immunohistochemistry (IHC) image for anti-Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody (ABIN185112) ABIN185112 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen ret...
Background publications Chan, Takai, Yamada et al.: "Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning." in: Oncogene, Vol. 12, Issue 6, pp. 1259-66, 1996 (PubMed).