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Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody

Details for Product No. ABIN185112
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Antigen
Synonyms arhgef8, net1a, xNET1, DKFZp459I0423, NET1, ARHGEF8, NET1A, 0610025H04Rik, 9530071N24Rik, AI604373, AU015857, Net1a, mNET1, wu:fb13g03, wu:fb25c11, zgc:92121
Epitope
C-Term
(7), (7), (5), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(76), (47), (44), (39), (37), (24), (24), (1), (1)
Host
Goat
(64), (10), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(33), (29), (19), (17), (6), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN185112
374.27 $
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Immunogen Peptide with sequence C-DKALSGGKRKETLV, from the C Terminus of the protein sequence according to NP_001040625.1, NP_005854.2.
Specificity This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Cross-Reactivity Mouse (Murine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name NET1 / ARHGEF8
Background Synonyms: NET1, ARHGEF8, NET1A, neuroepithelial cell transforming gene 1, Rho guanine nucleotide exchange factor (GEF) 8, guanine nucleotide regulatory protein (oncogene), neuroepithelial cell transforming 1, RP11-116G8.1, p65 Net1 proto-oncogene protein, small GTP-binding protein regulator
Gene ID 10276
NCBI Accession NP_001040625.1, NP_005854.2
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 85 kDa in Human Placenta and a very strong band at approx 40-50 kDa in Human Skeletal Muscle lysates after 0.1 µg/mL staining. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 67.7 kDa according to NP_001040625.1 and of 61.9 kDa according to NP_005854.2.
IHC: In paraffin embedded Human Tonsil shows staining of fibrous structures. Recommended concentration: 2.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Neuroepithelial Cell Transforming 1 (NET1) (C-Term) antibody ABIN185112 (2.5µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
General Chan, Takai, Yamada et al.: "Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning." in: Oncogene, Vol. 12, Issue 6, pp. 1259-66, 1996 (PubMed).

Validation Images
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