GIPC PDZ Domain Containing Family, Member 1 (GIPC1) antibody

Antigen: GIPC PDZ Domain Containing Family, Member 1 (GIPC1)

Synonyms: NIP, GIPC, IIP-1, TIP-2, SEMCAP, C19orf3, Hs.6454, MGC3774, GLUT1CBP, MGC15889, RGS19IP1, SYNECTIN, SYNECTIIN, gipc1, MGC56157, rgs19ip1, zgc:56157, zgc:77689, XGIPC, kermit, GIPC1, gipc3, iip-1, Kermit, semcap, glut1cbp, synectiin, TaxIP2, Semcap1, Glut1CIP, Rgs19ip1, Gipc, Rgs19

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185138

Additional Information

Alternative name: GIPC1 / NIP

Gene ID: 10755, 67903 (mouse), 83823 (rat)

Immunogen: C-GAIGDAKVGRY

Format: Affinity-purified

Description: All names and symbols: GIPC1, NIP, GIPC PDZ domain containing family, member 1, C19orf3, GIPC, GLUT1CBP, Hs.6454, IIP-1, MGC15889, MGC3774, RGS19IP1, SEMCAP, SYNECTIIN, TIP-2, GLUT1 C-terminal binding protein, IGF-1 receptor interacting protein 1, regulator of G-protein signalling 19 interacting protein 1, synectin. Official Symbol: GIPC1. RefSeq number(s): NP_005707.1, NP_974197.1, NP_974199.1, NP_974196.1, NP_974198.1, NP_974223.1. Gene ontology terms: protein binding, G-protein coupled receptor protein signaling pathway, soluble fraction, membrane fraction

Specificity: This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1). Human, Mouse, Rat

Synonyms: NIP, GIPC, IIP-1, TIP-2, SEMCAP, C19orf3, Hs.6454, MGC3774, GLUT1CBP, MGC15889, RGS19IP1, SYNECTIN, SYNECTIIN, gipc1, MGC56157, rgs19ip1, zgc:56157, zgc:77689, XGIPC, kermit, GIPC1, gipc3, iip-1, Kermit, semcap, glut1cbp, synectiin, TaxIP2, Semcap1, Glut1CIP, Rgs19ip1, Gipc, Rgs19

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, approx 24kDa also observed in human pancreas, kidney and skeletal muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). Recommended concentration: 0.03-0.1µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185138 staining (0.03µg/ml) of human HeLa cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: De Vries, Lou, Zhao et al.: "GIPC, a PDZ domain containing protein, interacts specifically with the C terminus of RGS-GAIP." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 21, pp. 12340-5, 1998 (PubMed).