Nucleoporin 85kDa (NUP85) antibody

Antigen: Nucleoporin 85kDa (NUP85)

Synonyms: Nup75, FLJ12549, Pcnt1, MGC127809

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185140

Additional Information

Alternative name: Pericentrin 1 / NUP85

Gene ID: 79902, 18541 (mouse)

Immunogen: C-ARAIIREGSLEGS

Format: Affinity-purified

Description: All names and symbols: NUP85, pericentrin 1, PCNT1, PCN, PCNT, frount, nucleoporin Nup75, nucleoporin 85kDa. Official Symbol: NUP85. RefSeq number(s): NP_079120

Specificity: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is NOT expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75KDa band (the expected size according to NP_079120) and not a 220kDa band (as reported for other anti-pericentrin antibodies). Human, Mouse, Rat 656 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 75kDa band observed in HeLa lysates (calculated MW of 75kDa according to NP_079120). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185140 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Doxsey, Stein, Evans et al.: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, 1994 (PubMed).