LIM Domain 7 (LMO7) antibody

Antigen: LIM Domain 7 (LMO7)

Synonyms: LOMP, FBX20, FBXO20, KIAA0858, Gm914, C78582, mKIAA0858, lmo7, MGC152922, zgc:152922, DKEY-180K21.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185145

Additional Information

Alternative name: LMO7 / FBXO20

Gene ID: 4008

Immunogen: ESTCVSASLPRS

Format: Affinity-purified

Description: All names and symbols: LMO7, FBXO20, LOMP, FBX20, KIAA0858, LIM domain only 7, LOMP protein, F-box protein Fbx20, F-box only protein 20, zinc-finger domain-containing protein. Official Symbol: LMO7. RefSeq number(s): AAD33924.1. Gene ontology terms: ubiquitin-protein ligase activity, ubiquitin ligase complex, protein ubiquitination

Specificity: Please note that the sequence we used differs one amino acid from the NCBI Reference Sequence (NP_005349.3). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 70-75kDa band and approx 30kDa band observed in Human Lung lysates (predicted MWs of 92kDa according to AAD33924.1 - however our result agrees with Putilina et al as below). A minor band of unknown identity was also consistently observed at approx 50kDa. Recommended for use at 0.5-2µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185145 staining (1µg/ml) of Human Lung lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Putilina, Jaworski, Gentleman et al.: "Analysis of a human cDNA containing a tissue-specific alternatively spliced LIM domain." in: Biochemical and biophysical research communications, Vol. 252, Issue 2, pp. 433-9, 1998 (PubMed).