Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A) antibody

Antigen: Par-6 Partitioning Defective 6 Homolog alpha (C. Elegans) (PARD6A)

Synonyms: Par6a, Par-6a, PARD6A

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185152

Additional Information

Alternative name: PAR6alpha / PARD6A

Gene ID: 50855

Immunogen: C-GSRIRGDGSGFSL

Description: All names and symbols: PARD6A, PAR6alpha, PAR-6, TAX40, PAR-6A, TIP-40, par-6 partitioning defective 6 homolog alpha (C.elegans), Tax interaction protein 40, par-6 (partitioning defective 6, C.elegans) homolog alpha. Official Symbol: PARD6A. RefSeq number(s): NP_058644. Gene ontology terms: transcription factor binding, viral life cycle, oncogenesis

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:8,000. Western Blot: Preliminary experiments gave no signal but low background in Human Brain and Pancreas extracts at up to 1µg/ml. Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration, 10µg/ml. Immunohistochemistry: In paraffin embedded Human Heart shows speckled staining on myocardial fibres in transverse section. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185152 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

ABIN185152 (5µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Johansson, Driessens, Aspenstroem: "The mammalian homologue of the Caenorhabditis elegans polarity protein PAR-6 is a binding partner for the Rho GTPases Cdc42 and Rac1." in: Journal of cell science, Vol. 113 ( Pt 18), pp. 3267-75, 2000 (PubMed).