PDZ and LIM Domain 4 (PDLIM4) antibody

Antigen: PDZ and LIM Domain 4 (PDLIM4)

Synonyms: RIL, Pdlim4, H-Rev18, Ril, MGC75597, MGC127818, GIG4, 0610007N03Rik, B230212B15Rik, cb764, hm:zeh0455, RAB13, MGC134259, PDLIM4

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185168

Additional Information

Alternative name: PDLIM4 / RIL

Gene ID: 8572, 30794 (mouse), 24915 (rat)

Immunogen: C-DVVAVYPNAKVELV

Format: Affinity-purified

Description: All names and symbols: RIL, PDLIM4, LIM domain protein, PDZ and LIM domain 4. Official Symbol: PDLIM4. RefSeq number(s): NP_003678.2. Gene ontology terms: tumor suppressor

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1/32,000. Western Blot: Approx 37kDa band observed in A431 lysates (predicted size of approx. 35kDa according to NP_003678). Recommended for use at 0.1-0.5µg/ml

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185168 staining (0.2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Bashirova, Markelov, Shlykova et al.: "The human RIL gene: mapping to human chromosome 5q31.1, genomic organization and alternative transcripts." in: Gene, Vol. 210, Issue 2, pp. 239-45, 1998 (PubMed).