Signal-Induced Proliferation-Associated Gene 1 (SIPA1) antibody

Antigen: Signal-Induced Proliferation-Associated Gene 1 (SIPA1)

Synonyms: SPA1, MGC17037, MGC102688

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185175

Additional Information

Alternative name: SPA1 / SIPA1

Gene ID: 6494

Immunogen: C-ASKQLGSPTADLA

Format: Affinity-purified

Description: All names and symbols: SIPA1, SPA1, MGC17037, signal-induced proliferation-associated gene 1, p130 SPA-1, GTPase-activating protein Spa-1. Official Symbol: SIPA1. RefSeq number(s): NP_694985.28, NP_006738.2. Gene ontology terms: GTPase activator, cell proliferation, signal transduction, peripheral plasma membrane protein

Specificity: Both variants represent identical protein (NP_694985.28 and NP_006738.2). Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Approx. 150kDa band observed in Human lysates of EBV-immortilized lymphoblastoid (calculated MW of 112kDa according to NP_694985.28 and NP_006738.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Kurachi et al, J Biol Chem. 1997 Oct 31,272(44):28081-8, PMID: 9346962). Recommended concentration: 1-3µg/ml. An additional band of 55-60kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when this product is used for other assays than Western blot.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185175 staining (1µg/ml) of Human EBV-immortilized lymphoblastoid lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Hattori, Tsukamoto, Nur-e-Kamal et al.: "Molecular cloning of a novel mitogen-inducible nuclear protein with a Ran GTPase-activating domain that affects cell cycle progression." in: Molecular and cellular biology, Vol. 15, Issue 1, pp. 552-60, 1995 (PubMed).