NADPH Oxidase Organizer 1 (NOXO1) antibody

Antigen: NADPH Oxidase Organizer 1 (NOXO1)

Synonyms: SNX28, P41NOX, P41NOXA, P41NOXB, P41NOXC, SH3PXD5, MGC20258, hslt, Snx28, 2310034C04Rik, NOXO1, cb18, sb:cb18, wu:fd09d09, noxo1, snx28, p41nox, p41noxa, p41noxb, p41noxc, sh3pxd5

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185195

Additional Information

Alternative name: NOXO1

Gene ID: 124056

Immunogen: RGCVDSVPHPTTEQ

Format: Affinity-purified

Description: All names and symbols: NOXO1, P41NOX, P41NOXA, P41NOXB, P41NOXC, MGC20258, NADPH oxidase organizer 1, regulatory protein P41NOX. Official Symbol: NOXO1. RefSeq number(s): NP_653204.1, NP_751907.1, NP_751907.1. Gene ontology terms: intracellular signaling cascade,

Specificity: This antibody is expected to recognise all three reported isoforms (as represented by NP_653204.1, NP_751907.1and NP_751908.1). Human 370 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave no signal but low background in Hela and Human Heart lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Immunohistochemistry: In paraffin embedded Human Kidney Cortex shows cytoplasmic stain in epithelial cells of some PCT. Recommended concentration, 10-20µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Signaling, Metabolism, Transcription Factors, Enzymes

Restrictions: For Research Use only

Images

ABIN185195 (10µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Banfi, Clark, Steger et al.: "Two novel proteins activate superoxide generation by the NADPH oxidase NOX1." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3510-3, 2003 (PubMed).