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Four and A Half LIM Domains 2 (FHL2) antibody
| Antigen | Four and A Half LIM Domains 2 (FHL2) |
| Synonyms | DRAL, AAG11, SLIM3, C76204, FHL2, MGC137365, BFGF, FGFB, HBGF-2, Fgfb, bFGF, Fgf-2, FGF2, nudt6, fgf2, MGC52602, FGF-2, bfgf, fgfb, fgf-2, xfgf2, hbgh-2, HBGF2, LOC100227798 |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN185198 |
| Quantity | 100 ug |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | FHL2 |
| Gene ID | 2274, 14200 (mouse), 63839 (rat) |
| Immunogen | RDDILCPDCGKDI |
| Format | Affinity-purified |
| Description | All names and symbols: FHL2, DRAL, AAG11, SLIM3, four and a half LIM domains 2, down-regulated in rhabdomyosarcoma LIM protein, aging-associated gene 11, four and a half LIM-domain protein 2. Official Symbol: FHL2. RefSeq number(s): NP_001441.4, NP_963849.1, NP_963851.2, NP_001034581.1. Gene ontology terms: oncogenesis, nucleus |
| Specificity | NP_001441.4, NP_963849.1, NP_963851.2 and NP_001034581.1 represent varients of the same protein. Human, Mouse, Rat 279 aa |
Application Details
| Application Notes | Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx 30kDa band observed in Human Heart lysates (calculated MW of 32.2kDa according to NP_001441.4). Recommended concentration: 1-3µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Research Area | Chromatin and Nuclear Signaling, Transcription Factors |
| Restrictions | For Research Use only |
Images
| ABIN185198 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publications
| Publications |
Morgan, Madgwick: "Slim defines a novel family of LIM-proteins expressed in skeletal muscle." in: Biochemical and biophysical research communications, Vol. 225, Issue 2, pp. 632-8, 1996 (PubMed).
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Alternatives
Alternatives for antigen "Four and A Half LIM Domains 2 (FHL2)", type "Antibodies"
| Hosts | Rabbit (34), Mouse (6), Goat (3) |
| Reactivities | Human (39), Mouse (Murine) (18), Rat (Rattus) (17), Cow (Bovine) (2), Dog (Canine) (2), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1) |
| Applications | Western Blotting (WB) (24), Immunofluorescence (IF) (21), Flow Cytometry (FACS) (20), ELISA (7), Enzyme Immunoassay (EIA) (5), Immunohistochemistry (IHC) (4), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Immunoprecipitation (IP) (3), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1) |
| Conjugates | Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitopes | C-Term (3), N-Term (2) |
