DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5) antibody

Antigen: DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5)

Synonyms: p68, HLR1, G17P1, HUMP68, DKFZp434E109, DKFZp686J01190, Hlr1, MGC118083, 2600009A06Rik, wu:fa56a07, wu:fb11e01, wu:fb16c10, wu:fb53b05, ddx5, MGC76265, DDX5, DKFZp459M043

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185199

Additional Information

Alternative name: DDX5 / p68 RNA helicase

Gene ID: 1655, 13207 (mouse)

Immunogen: C-PMIGYPMPTGYSQ

Format: Affinity-purified

Description: All names and symbols: DDX5, P68, HLR1, G17P1, HUMP68, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kDa), RNA-dependent ATPase, DEAD/H box-5 (RNA helicase, 68kD), DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 5 (RNA helicase, 68kD). Official Symbol: DDX5. RefSeq number(s): NP_004387.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 70kDa band observed in human epidermoid carcinoma A431 lysates (calculated MW of 69.1kDa according to NP_004387). Recommended concentration: 0.11-0.3µg/ml. Immunofluorescence: A customer has reported successful staining at a dilution of 1:100 on HT-1080 cells with a Rhodamine conjugated secondary.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185199 staining (0.5µg/ml) of A431 cell lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Iggo, Gough, Xu et al.: "Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 16, pp. 6211-4, 1989 (PubMed).