A Kinase (PRKA) Anchor Protein 3 (AKAP3) antibody

Antigen: A Kinase (PRKA) Anchor Protein 3 (AKAP3)

Synonyms: CT82, SOB1, FSP95, PRKA3, AKAP110, Prka3, AKAP3, MGC93915

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185200

Additional Information

Alternative name: AKAP3 / SOB1

Gene ID: 10566

Immunogen: C-TPLQLLDWLMVNL

Format: Affinity-purified

Description: All names and symbols: AKAP3, SOB1, FSP95, PRKA3, AKAP110, A kinase (PRKA) anchor protein 3, fibrousheathin 1, sperm oocyte binding protein 1. Official Symbol: AKAP3. RefSeq number(s): NP_006413. Gene ontology terms: protein kinase A anchor protein activity, acrosome reaction, fertilization (sensu Animalia), cell motility

Specificity: Human 853 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western Blot: Approx 90kDa band observed in Human Testis lysates (calculated MW of 95kDa according to NP_006413). Recommended concentration: 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185200 (1µg/ml) staining of Human Testis lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publications

Publications: Lefevre, Duquenne, Rousseau-Merck et al.: "Cloning and characterization of SOB1, a new testis-specific cDNA encoding a human sperm protein probably involved in oocyte recognition." in: Biochemical and biophysical research communications, Vol. 259, Issue 1, pp. 60-6, 1999 (PubMed).