PITPNM Family Member 3 (PITPNM3) antibody

Antigen: PITPNM Family Member 3 (PITPNM3)

Synonyms: NIR1, CORD5, RDGBA3, MGC157740, MGC157741

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Conjugate: Un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185211

Additional Information

Alternative name: NIR1 / PITPNM3

Gene ID: 83394

Immunogen: C-SWARGPPKFESVP

Description: All names and symbols: NIR1, PITPNM family member 3, PYK2 N-terminal domain-interacting receptor 1, RDGBA3. Official Symbol: PITPNM3. RefSeq number(s): NP_112497

Specificity: Human 974 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 50kDa band in Human Heart lysates after 0.1µg/ml antibody staining. The 50kDa band was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_112497. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Cerebral Cortex shows strong staining of cytoplasm in several neuronal cells. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185211 (5µg/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Lev, Hernandez, Martinez et al.: "Identification of a novel family of targets of PYK2 related to Drosophila retinal degeneration B (rdgB) protein." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 2278-88, 1999 (PubMed).