Intraflagellar Transport Protein 74 Homolog (IFT74) antibody

Antigen: Intraflagellar Transport Protein 74 Homolog (IFT74)

Synonyms: CMG1, CCDC2, CMG-1, FLJ22621, MGC111562, Cmg1, Ccdc2, 1700029H06Rik, MGC94371, DKFZp469F1522

Clonality: Polyclonal

Host: Goat

Reactivity: Human, Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN185213

Additional Information

Alternative name: CMG1 / CCDC2 / IFT74

Gene ID: 80173, 313365 (rat)

Immunogen: C-KTIVDALHSTSGN

Format: Affinity-purified

Description: All names and symbols: IFT74, CCDC2, CMG-1, FLJ22621, MGC111562, intraflagellar transport 74 homolog (Chlamydomonas), capillary morphogenesis protein 1, coiled-coil domain containing 2,. Official Symbol: IFT74. RefSeq number(s): NP_079379.1, AAK77221.1

Specificity: Human, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Approx 70-75kDa band observed in lysates of cell line HEK293 (calculated MW of 69.2kDa according to AAK77221.1, but 51.4kDa according to NP_079379.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Momeni et al, BMC Neurol. 2006 Dec 13,6:44., PMID: 17166276). Recommended concentration: 0.3-1µg/ml. Immunohistochemistry: In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Recommended concentration, 5-10µg/ml. Immunofluorescence: successfully used at 2.5mg/ml to stain rat cortical neurons (see picture and PMID: 17166276). IF picture: A. ABIN185213 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B. Secondary antibody alone gave no signal using consistent gain and offset settings. (Detected by fluorescence, Data were kindly provided by Dr. Bryan Traynor and published in Momeni P. et al., BMC Neurol. 2006 Dec 13, 6:44.) Figure 2: A. ABIN185213 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor. See Application Notes!)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185213 staining (0.3µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Figure 1: ABIN185213 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: see Application Notes

Publications

Publications: Bell, Mavila, Salazar et al.: "Differential gene expression during capillary morphogenesis in 3D collagen matrices: regulated expression of genes involved in basement membrane matrix assembly, cell cycle progression, cellular differentiation and G-protein signaling." in: Journal of cell science, Vol. 114, Issue Pt 15, pp. 2755-73, 2001 (PubMed).