WD Repeat and FYVE Domain Containing 1 (WDFY1) antibody

Antigen: WD Repeat and FYVE Domain Containing 1 (WDFY1)

Synonyms: WDF1, FENS-1, ZFYVE17, Jr1, KIAA1435, mKIAA1435, 1700013B03Rik, 1700120F24Rik, MGC93914, MGC109909, zgc:101764, zgc:109909, MGC137087

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN185214

Additional Information

Alternative name: FENS1 / WDFY1

Gene ID: 57590

NCBI Acession: NP_065881.1

Immunogen: Peptide with sequence VVGCSLATGFSPH, from the C Terminus of the protein sequence according to NP_065881.1.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Format: Liquid

Description: This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127). Principal Names: ZFYVE17, phosphoinositide-binding protein SR1, WD40 and FYVE domain containing 1, WD repeat and FYVE domain containing 1, KIAA1435, FENS-1, WDF1, WDFY1. Official Symbol: WDFY1

Specificity: This antibody is expected to recognise both reported isoforms (as represented by NP_065881 and NP_848127) Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave no signal but low background in Human Liver and HepG2 lysates at up to 1µg/ml. IHC: In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatoicytes. Recommended concentration, 4-6µg/ml.

Concentration: 100µg/200µl

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Publications

Ridley, Ktistakis, Davidson et al.: "FENS-1 and DFCP1 are FYVE domain-containing proteins with distinct functions in the endosomal and Golgi compartments." in: Journal of cell science, Vol. 114, Issue Pt 22, pp. 3991-4000, 2001 (PubMed).