Actin-Like 6B (ACTL6B) antibody

Antigen: Actin-Like 6B (ACTL6B)

Synonyms: ACTL6, BAF53B, Actl6, ArpNa, Baf53b, MGC139460, MGC110167, zgc:110167

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN185217

Additional Information

Alternative name: BAF53A and BAF53B

Gene ID: 51412, 86

Immunogen: YEEGGKQCVERKCP

Format: Affinity-purified

Description: All names and symbols: BAF53A, BAF53B, ACTL6, BAF53a, BAF53b, actin-like 6A, actin-like 6B, hArpN alpha, actin-related protein, ACTL6A, ACTL6B. Official Symbol: ACTL6A / ACTL6B. RefSeq number(s): NP_057272, NP_004292. Gene ontology terms: structural constituent of cytoskeleton, establishment and/or maintenance of chromatin architecture, nucleus

Specificity: This antibody is expected to recognise both BAF53A and BAF53B, which are almost identical. Human, Mouse, Rat 426 aa

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 48kDa band observed in Hela lysates (predicted MW of 49kDa according to NP_057272, NP_004292). Recommended for use at 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN185217 staining (1µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Publications

Publications: Harata, Mochizuki, Mizuno: "Two isoforms of a human actin-related protein show nuclear localization and mutually selective expression between brain and other tissues." in: Bioscience, biotechnology, and biochemistry, Vol. 63, Issue 5, pp. 917-23, 1999 (PubMed).